Activités de recherche, techniques  : 

•  Etude des réponses immunitaires de la vigne, de leur stimulation par des SDP (Stimulateurs de Défenses des Plantes) en stratégie de biocontrôle et de facteurs et leviers susceptibles de les moduler, via leurs effets sur la physiologie de la plante (facteurs environnementaux, biostimulation, …).      
•   Développement d’outils pour le criblage et l’étude de biostimulants actifs sur vigne : caractérisation des effets sur le développement de la plante (phénotypage aérien / racinaire), sa physiologie (expression génique, analyse ciblée /globale de métabolites) et sa résistance à des stress abiotiques (dont hydrique).
• *Etude de l’utilisation combinée de biosolutions (SDP, biostimulants) afin de les intégrer au vignoble dans une démarche de transition agroécologique.

Activités d’enseignement :

•  Enseignements en biologie et physiologie de la vigne, pathologies de la vigne, stratégies de protection du vignoble, matériel végétal en viticulture, écophysiologie.
• Formation initiale:  Licence des Sciences de la vigne (L3) - Licence pro Conduite Stratégique de l’Exploitation Vitivinicole - Master Vigne Vin Terroir - Diplôme National d’Œnologue (DNO)
• Formation continue:  Diplômes universitaires Vin Culture Œnotourisme (présentiel / co-modal) et Expertise en Dégustation Professionnelle des Vins                                                                                                   
• Formation courte (en anglais) : Terroir Vineyard Management
• Mastère OIV  MSc in Wine Management

Publications :

Trouvelot S., Lemaître Guillier C., Vallet J., Jacquens L., Douillet A., Harir M., Larignon P., Roullier-Gall C., Schmitt-Kopplin P., Adrian M., Fontaine. F. (2023). Sodium arsenite-induced changes in the wood of Esca-diseased grapevine at cytological and metabolomic levels. Front. Plant Sci. 14. doi: 10.3389/fpls.2023.1141700

Fernandez O., Lemaître-Guillier C., Songy A., Robert-Siegwald G., Lebrun M.H., Schmitt-Kopplin P., Larignon P., Adrian M., Fontaine F.   (2023). The combination of both heat and water stresses may worsen
Botryosphaeria dieback symptoms in grapevine. Plants 12(4), 753. https://doi.org/10.3390/plants12040753

Jacquens L., Trouvelot S., Lemaitre-Guillier C., Krzyzaniak Y., Clément G., Citerne S., Mouille G., Moreau E. Héloir M.C., Adrian M. (2022). Biostimulation can prime elicitor induced resistance of grapevine leaves to downy mildew. Front. Plant Sci. 13:998273. doi: 10.3389/fpls.2022.998273.

Lemaitre-Guillier C., Chartier A., Dufresne C., Douillet A., Cluzet S., Valls J., Aveline N., Daire X., Adrian M. (2022). Elicitor-Induced VOC Emission by Grapevine Leaves: Characterisation in the Vineyard. Molecules 27, 6028. https://doi.org/10.3390/molecules27186028

Mondello V., Lemaître-Guillier C., Trotel-Aziz P., Gougeon R., Acedo, A., Schmitt-Kopplin P., Adrian M., Pinto C., Olivier Fernandez O., and Florence Fontaine F. (2022) Assessment of a New Copper-Based Formulation to Control Esca Disease in Field and Study of Its Impact on the Vine Microbiome, Vine Physiology and Enological Parameters of the Juice. Journal of Fungi 8, 2, 151; https://doi.org/10.3390/jof8020151

Lemaitre-Guillier C., Dufresne C., Chartier A., Cluzet S., Valls J., Jacquens L., Douillet A., Aveline N., Adrian M., Daire X. (2021). VOCs Are Relevant Biomarkers of Elicitor-Induced Defences in Grapevine. Molecules 26, 4258. https://doi.org/10.3390/molecules26144258

Krzyzaniak Y., Cointault F., Loupiac C., Bernaud E., Ott F., Salon C., Laybros A., Han S., Héloir M.C., Adrian M., Trouvelot S. (2021). In situ phenotyping of grapevine root system architecture by 2D or 3D imaging: advantages and limits of three cultivation methods. Frontiers Plant Sci. 12, 638688,  https://doi.org/10.3389/fpls.2021.638688

Negrel J., Klinguer A., Adrian M. (2021). In vitro inhibition of shikimate hydroxycinnamoyltransferase by acibenzolar acid, the first metabolite of the plant defence inducer acibenzolar-S-methyl. Plant Physiology and Biochemistry 163, 119-127. Doi 10.1016/j.plaphy.2021.03.050

Moret F., Delorme G., Clément G., Grosjean C., Lemaître-Guillier C., Trouvelot S., Adrian M.*, Fontaine F. (2021).  Esca‐affected grapevine leaf metabolome is clone‐ and vintage‐dependent. Physiologia Plantarum. https://doi.org/10.1111/ppl.13254. *co-dernier auteur

Lemaitre-Guillier C., Fontaine F., Roullier-Gall C., Harir M., Magnin-Robert M., Clément C., Trouvelot S., Gougeon R.D., Schmitt-Kopplin P., Adrian M. (2020). Cultivar- and wood area-dependent metabolomic fingerprints of grapevine infected by Botryosphaeria dieback. Phytopathology 110, 11, 1821-37. doi: 10.1094/PHYTO-02-20-0055-R  Photo de l’article en couverture de page de l’article

Moret F., Clément G., Grosjean C., Lemaître-Guillier C., Morvan G., Trouvelot, Adrian M., Fontaine F. (2020). Metabolic fingerprint of Chardonnay leaves affected by esca disease is clone and year dependent. Phytopathologia Mediterranea, 11th Special issue on Grapevine Trunk Diseases, 59, 3, 595-603. doi: 10.14601/Phyto-11170.

Héloir M.C., Adrian M., Brulé D., Claverie J., Cordelier S., Daire X., Dorey S., Gauthier A., Lemaître-Guillier C., Negrel J., Trda L., Trouvelot S., Vandelle E., Poinssot B. (2019). Grapevine recognition of elicitors: from the MAMP/DAMP perception to induced resistance. Frontiers in Plant Science doi: 10.3389/fpls.2019.01117

Li Y. Héloir M.C., Zhang X., Geissler M., Trouvelot S., Jacquens L., Henkel M., Su X., Fang X., Wang Q., Adrian M. (2019). Surfactin and fengycin contribute to the protection of a Bacillus subtilis strain against grape downy mildew by both direct effect and defense stimulation. Molecular Plant Pathology 20, 8, 1037-1050. doi: 10.1111/mpp.12809. 

Moret F.- Lemaître-Guillier C., Grosjean C., Clément C., Coelho C., Negrel, J. Jacquens L., Morvan G., Mouille G., Trouvelot, Fontaine F., Adrian M. (2019). Clone-dependent Expression of Esca Disease Revealed by Leaf Metabolite Analysis. Frontiers in Plant Science 9:1960. doi: 10.3389/fpls.2018.01960. eCollection 2018.

Paris F., Trouvelot S., Jubien M., Lecollinet G., Joubert J.M., Chiltz A., Héloir M.C., Negrel J., Adrian M., Legentil L., Daire X., Ferrières V. (2019). Hydrophobized laminarans as new biocompatible anti-oomycete compounds for grapevine protection. Carbohydrate Polymers 225, 115224. 10.1016/j.carbpol.2019.115224. 

Sbardelotto De Bona G., Adrian M., Negrel J., Chiltz A., Klinguer A., Poinssot B., Héloir M.C., Angelini E., Vincenzi S., Bertazzon N. (2019). Dual mode of action of grape cane extracts against Botrytis cinerea. Journal of Agricultural and Food Chemistry 67, 19, 5512-20. doi : 10.1021/acs.jafc.8b07098.

Brulé D., Villano C. Davies L., Trdá L., Claverie J., Héloir M.C., Chiltz A., Adrian M., Darblade B., Tornero P., Stransfeld L., Boutrot F., Zipfel C., Dry I., Poinssot, B. (2019). The grapevine (Vitis vinifera) LysM receptor kinases VvLYK1-1 and VvLYK1-2 mediate chitooligosaccharide-triggered immunity. Plant Biotechnology Journal. doi.org/10.1111/pbi.13017.

Héloir M.C., Kim Khiook I. L., Lemaître-Guillier C., Clément G., Jacquens L., Bernaud E., Trouvelot S., Adrian M. (2018). Assessment of the impact of PS3-induced resistance to downy mildew on grapevine physiology. Plant Physiology and Biochemistry. 133-134-141. 10.1016/j.plaphy.2018.10.030

Krzyzaniak Y., Trouvelot S., Negrel J., Cluzet S., Valls J., Richard T., Jacquens L., Klinguer A., Chiltz A., Adrian M., Héloir M.C. (2018). A plant extract acts both as a resistance inducer and an oomycide against grapevine downy mildew. Frontiers in Plant Science 9:1085. doi: 10.3389/fpls.2018.01085.

Krzyzaniak Y., Negrel J., Lemaitre-Guillier C., Klinguer A., Clément G., Mouille G., Trouvelot S., Héloir M.C., Adrian M. (2018). Combined enzymatic and metabolic analysis of grapevine cell responses to elicitors. Plant Physiology and Biochemistry 123, 141–148. DOI 10.1016/j.plaphy.2017.12.013.

Adrian M., Lucio M., Roullier-Gall C., Héloir M.C., Trouvelot S., Daire X., Kanawati B., Lemaître-Guillier C., Poinssot B., Gougeon R. & Schmitt-Kopplin P. (2017). Metabolic fingerprint of PS3-induced resistance of grapevine leaves against Plasmopara viticola revealed differences with elicitor-triggered defenses. Frontiers in Plant Science, 8:101. doi: 10.3389/fpls.2017.00101

Hao Z., Van Tuinen D., Wipf D., Fayolle L., Chataignier O., Li X., Chen B., Gianinazzi S., Gianinazzi-Pearson V., Adrian M. (2017). Biocontrol of grapevine aerial and root pathogens by Paenibacillus sp. strain B2 and paenimyxin in vitro and in planta. Biological Control 109, 42-50.

Magnin-Robert M., Adrian M., Trouvelot S., Spagnolo A., Jacquens L., Letousey P., Harir M., Roullier-Gall C., Clément C., Schmitt-Kopplin P., Vallat A., Abou-Mansour E., Fontaine F. (2017). Alterations in grapevine leaf metabolism occur prior to esca apoplexy appearance. Molecular Plant Microbe Interaction 30(12):946-959. https://doi.org/10.1094/MPMI-02-17-0036-R.

Lemaître-Guillier C., Hovasse A., Schaeffer-Reiss C., Recorbet G., Poinssot B., Trouvelot S., Daire X., Adrian M., Héloir MC. (2017). Proteomics towards the understanding of elicitor induced resistance of grapevine against downy mildew. Journal of Proteomics 156:113-125. doi: 10.1016/j.jprot.2017.01.016.

Jeudy C., Adrian M., Baussart B., Bernard C., Bernaud E., Bourion V., Busset H., Cabrera L., Cointault F., Han S., Lamboeuf M., Moreau D., Pivato B., Prudent M., Trouvelot S., Truong H.N., Vernoud V., Voisin A.S., Wipf D., Salon C. (2016). RhizoTubes as a new tool for high throughput imaging of plant root development and architecture: test, comparison with pot grown plants and validation. Plant Methods 12, 31. doi:  10.1186/s13007-016-0131-9

Paris F., Krzyzaniak Y., Gauvrit C., Jamois F., Domergue F., Joubès J., Ferrières V., Adrian M., Legentil L., Daire X., Trouvelot S. (2016). An ethoxylated surfactant enhances the penetration
of the sulfated laminarin through leaf cuticle and stomata, leading to increased induced resistance against grapevine downy mildew. Physiologia plantarum 156, 3, 338-350. doi:10.1111/ppl.12394

Gamm M., Héloir M.C., Adrian M. (2015). Trehalose and trehalose-6-phosphate induce stomatal movements and interfere with ABA-induced stomatal closure in grapevine. Journal International des Sciences de la Vigne et du Vin 49,165-171. doi.org/10.20870/oeno-one.2015.49.3.84

Trouvelot S., Bonneau L., Redecker D., van Tuinen D., Adrian M., Wipf D. (2015). Arbuscular mycorrhiza symbiosis in viticulture. A review." Agronomy for Sustainable Development 35,4, doi :10.1007/s13593-015-0329-7.

Guillier C., Gamm M., Lucchi G., Truntzer C., Pecqueur D., Adrian M., Héloir M.C. (2015). Towards the identification of two glycoproteins involved in the stomatal deregulation of downy mildew infected grapevine leaves. Molecular Plant Microbe Interaction 28,11. 1227-1236. https://doi.org/10.1094/MPMI-05-15-0115-R.

Chalal M., Winkler J.B., Gourrat K., Trouvelot S., Adrian M., Schnitzler J.P., Jamois F., Daire X. (2015). Sesquiterpene VOCs are markers of elicitation by sulfated laminarine in grapevine. Frontiers in Plant Science 6:350. doi: 10.3389/fpls.2015.00350.

Trouvelot S., Héloir M.C., Poinssot B., Gauthier A., Paris F., Guillier C., Combier M., Tdra L., Daire X., Adrian M. (2014). Carbohydrates in plant immunity and plant protection: roles and potential application as foliar sprays. Frontiers in Plant Science doi: 10.3389/fpls.2014.00592.

Lachhab N., Sanzani M., Adrian M., Chiltz A., Balacey S., Boselli M., Ippolito A., Poinssot B. (2014). Soybean and casein hydrolysates induce grapevine immune responses and resistance against Plasmopara viticola. Frontiers in Plant Science 5, 716.  doi: 10.3389/fpls.2014.00716.

Chalal M., Klinguer A., Echairi A., Meunier P., Vervandier-Fasseur D., Adrian M. (2014). Antimicrobial activity of resveratrol analogues. Molecules 19, 7679-7688. 10.3390/molecules19067679.

Trdá L., Fernandez O., Boutrot F., Héloir M.C., Kelloniemi J., Daire X., Adrian M., Clément C., Zipfel C., Dorey S., Poinssot B. (2014). The grapevine flagellin receptor VvFLS2 differentially recognizes flagellin-derived epitopes from the endophytic growth-promoting bacterium Burkholderia phytofirmans and plant pathogenic bacteria. New Phytologist DOI: 10.1111/nph.12592.

Kim Khiook I.L., Schneider C., Heloir M.C., Bois B., Daire X., Adrian M., Trouvelot S. (2013). Image analysis methods for assessment of H2O2 production and Plasmopara viticola development in grapevine leaves: Application to the evaluation of resistance to downy mildew. Journal of Microbiological Methods, 28, 95(2), 235-244. doi: 10.1016/j.mimet.2013.08.012.

Steimetz E., Trouvelot S., Gindro K., Bordier A., Poinssot B., Adrian M., Daire X. (2012). Influence of leaf age on induced resistance in grapevine against Plasmopara viticola. Physiological and Molecular Plant Pathology 79, 89-96. DOI  10.1016/j.pmpp.2012.05.004. 

Adrian M., Jeandet P. (2012). Effects of resveratrol on the ultrastructure of Botrytis cinerea conidia and biological significance in plant / pathogen interactions. Fitoterapia 83, 1345-1350. DOI: 10.1016/j.fitote.2012.04.004.

Gamm M., Héloir M.C., Bligny R., Vaillant-Gaveau N., Trouvelot S., Alcaraz G., Frettinger P., Clément C., Pugin A., Wendehenne D., Adrian M. (2011). Changes in carbohydrate metabolism in Plasmopara viticola -infected grapevine leaves. Molecular Plant-Microbe Interaction 24, 9, 1061-1073. doi:10.1094/MPMI-02-11-0040.

Gamm M., Héloir M.C., Kelloniemi J., Poinssot B., Wendehenne D., Adrian M. (2011). Identification and Validation of reference genes for gene expression normalization ingrapevine. Molecular Genetics and Genomics DOI 10.1007/s00438-011-0607-2.

Allègre M., Héloir M.C., Trouvelot S., Daire X., Pugin A., Wendehenne D., Adrian M., (2009).
Are Grapevine Stomata Involved in the Elicitor-Induced Protection Against Downy Mildew?
Molecular Plant-Microbe Interactions 22, 977-986.

Allègre M., Daire X., Héloir M.C., Trouvelot S., Mercier L., Adrian M., Pugin A. (2007). Stomatal deregulation in Plasmopara viticola-infected grapevine leaves. New Phytologist 173, 832-840.

Lesniewska E. / Adrian M., Klinger A., Pugin A. (2004). Cell wall modification in grapevine cells in response to UV stress investigated by atomic force microscopy. Ultramicroscopy 100, 171-178.

Borie B., Jeandet P., Bessis R., Adrian M. (2003). Comparison of resveratrol and stilbene synthase mRNA production from grapevine leaves treated with biotic and abiotic phytoalexin elicitors. American Journal of Enology and Viticulture 55, 1, 60-64.

Poussier M., Guilloux-Benatier M., Torres M., Heras E., Adrian M. (2003). Influence of different maceration techniques and microorganism enzymatic activities on wine stilbene content. American Journal of Enology and Viticulture 54,4, 261-266.

Aziz A. /Poinssot B., Daire X., Adrian M., Bézier A., Joubert J.M., Pugin A. (2003). Laminarin elicits defence responses in grapevine and induced protection against Botrytis cinerea and Plasmopara viticola. Molecular Plant Microbe Interaction 16,12, 1118-1128.

Poinssot B., Vandelle E., Bentéjac M., Adrian M., Levis C., Brygoo Y., Garijn J., Sicilia F., Coutos-Thévenot P., Pugin A. (2003). The endopolygalacturonase 1 from Botrytis cinerea activates grapevine defence reactions unrelated to its enzymatic activity. Molecular Plant Microbe Interaction 16, 6, 553-564.

Jeandet P., Douillet-Breuil A.C., Bessis R., Debord S., Sbaghi M., Adrian M. (2002). Phytoalexins from the Vitaceae : biosynthesis, phytoalexin gene expression in transgenic plants, antifungal activity and metabolism. Journal of Agricultural and Food Chemistry 50, 10, 2731-2741.

Jeandet P., Adrian M., Breuil A.C., Sbaghi M., Debord S., Bessis R., Weston L.A. and Harmon R. (2000). Chemical induction of phytoalexin synthesis in grapevines: Application to the control of grey mould (Botrytis cinerea Pers.) in the vineyard. Acta Horticulturae 528, 591-596.

Moriartry J.M., Harmon R., Weston L.A., Bessis R., Breuil A.C., Adrian M., Jeandet P. (2001). Resveratrol content of two Californian table grape cultivars. Vitis 40,1, 43-44.

Adrian M., Jeandet P., Douillet-Breuil A.C., Tesson L., Bessis R. (2000). Stilbene content of mature Vitis vinifera berries in response to UV-C elicitation. Journal of Agricultural and Food Chemistry, 48, 12, 6103-6105.

Adrian M., Jeandet P., Breuil A.C., Levite D., Debord S., Bessis R. (2000). Assay of resveratrol and derivative stilbenes in wines by direct injection high performance liquid chromatography. American Journal of Enology and Viticulture 51,1, 37-41.

Douillet-Breuil A.C., Jeandet P., Adrian M., Bessis R. (1999). Changes in the phytoalexin content of various Vitis spp. in response to UV-C elicitation. Journal of Agricultural and Food Chemistry 47, 10, 4456-4461.

Breuil A.C., Jeandet P., Adrian M., Chopin F., Pirio N., Meunier P., Bessis R. (1999). Characterization of a pterostilbene dehydrodimer produced by laccase of Botrytis cinerea. Phytopathology, 89, 4, 298-302.

Breuil A.C., Adrian M., Pirio N., Weston L.A., Meunier P., Bessis R., Jeandet P. (1998). Metabolism of stilbene phytoalexins by Botrytis cinerea : characterization of a resveratrol dehydrodimer. Tetrahedron Letters, 39, 537-540.

Adrian M., Rajaei H., Jeandet P., Veneau J., Bessis R. (1998). Resveratrol oxidation in Botrytis cinerea conidia. Phytopathology, 88, 5, 472-476.

Jeandet P., Breuil A.C., Adrian M., Weston L.A., Meunier P., Maume G., Bessis R. (1997). HPLC analysis of stilbene phytoalexins coupling photodiode array detection and fluorimetry.  Analytical Chemistry, 69, 24, 5172-5177.

Adrian M., Jeandet P., Veneau J., Weston L.A., Bessis R. (1997). Biological activity of resveratrol, a stilbenic compound from grapevines, on Botrytis cinerea, the causal agent for gray mold. Journal of Chemical Ecology, 23, 1689-1702.

Adrian M., Jeandet P., Joubert J.M., Bessis R. (1996). Induction of phytoalexin (resveratrol) synthesis in grapevine leaves treated with aluminum chloride (AlCl3). Journal of Agricultural and Food Chemistry, 44, 8, 1979-1981.

Adrian M., Fournioux J.C., Bessis R. (1995). In vitro morphogenesis of grapevine (Vitis vinifera L.) originated from anticipated or latent buds. Vitis, 35, 3, 107-111.

Principaux contrats :

Projets pédagogiques :

•  Projet VINUM « Formations numériques des filières agricoles, viticoles, forestières » (2022-25). Responsable pour l’IUVV-uB de ce projet porté par UNIT. Financement France 2030, AMI « Compétences et Métiers d’Avenir » - Banque des territoires. https://unit.eu/nos-projets/vinum
• Production de vidéos pédagogiques sur l’ampélographie traditionnelle (2021). Responsable de ce projet collaboratif (GEST, Institut Agro Montpellier, IFV, INRAE Vassal - Bourgogne Live Prod, Brainchild Motion&Movies).
• MOOC OWU2 Open Wine University 2 (2017-18) : Création et responsabilité, avec l’appui de la Direction Numérique de l’uB et du GIP BVV de ce projet ISITE-BFC. Production en français traduite en anglais, espagnol et chinois, prochainement en japonais.

Projets de recherche :

• Programme Prioritaire de Recherche « Cultiver et protéger autrement » VITAE (2021-27). « Cultiver la vigne sans pesticides : vers des socio-écosystèmes viticoles agroécologiques ». France 2030 - ANR. Co-pilotage de ce projet avec F. Delmotte (INRAE Bordeaux) et H. Hannin (Institut Agro Montpellier) et responsable du WP Biocontrôle.                                                                                  
• Projet INRAE SPE PhytoStim (2019-21). « La biostimulation : un levier d’augmentation de l’efficacité des SDP via ses effets sur la vigne ? » Contribution scientifique à ce projet porté par MF Corio-Costet (INRAE Bordeaux).                                                                                                                        
• Projet ANR Chaire Industrielle « GTD Free » (2016-20). « Influence of environmental factors and cropping practices on grapevine trunk diseases expression and plant-microbiota interaction ». ANR-Jas Hennessy & Co. Responsable pour l’équipe de ce projet porté par l’UMR SAVE (Inrae Bordeaux-BSA).                                                                                   
•  Projet FUI Iris+ (2015-18). « Solutions innovantes pour la santé de la vigne et du blé associant applications de biostimulants et SDP à des technologies d’imagerie et d’agroéquipements ».  Responsable scientifique de ce projet porté par Arysta Life Sciences.                                                      
• Projets Région BFC / Feder                                                                         
• Prestations de service et projets de recherche et développement avec des partenaires privés

Liens externes (Links) :

https://iuvv.u-bourgogne.fr/ 

https://www.inrae.fr/actualites/cultiver-vigne-pesticides-lancement-du-projet-vitae